Evaluation of the genotoxic effect of tartrazine using a metabolic activation system in human lymphocyte culture under cytokinetic block conditions

The expansion of the spectrum use food additives and, in particular, dyes (FD), increases risk increasing human exposure to genotoxicants. Since real life, not pure substances with proven genetic safety are contact a person, but complex mixtures unknown composition, even minor impurities which can become an additional source or modifier genome instability effects. Of particular concern this aspect synthetic FD azo and diazo compounds be transformed by intestinal microflora some forms purpose work is evaluate genotoxic effects 02 mg/mL Tartrazine (E102) purchased retail network micronucleus test on blood cells cultured under cytokinetic block conditions parallel presence without rat S9 hepatocyte metabolic activation system.
 Genotoxic were found cultures at 0.00002560,00064 0.4 tartrazine, 0.0000256 mg/mL, 0,000128 0.16 tartrazine. For first time, dose-dependent suppression mitotic proliferative activity lymphocytes induced tested tartrazine sample was revealed, as well U-shaped curve frequency apoptosis. data obtained indicate studied sample.
 We discuss necessity create system for evaluation from network.